Retinal pigment epithelial cells can be cultured on fluocinolone acetonide treated nanofibrous scaffold

Retinal pigment epithelial cells can be cultured on fluocinolone acetonide treated nanofibrous scaffold

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Engineered tissue currently Rectangular Plate lacks requisite capacity to sustain cell viability and functionality.Here we demonstrate that human RPE cell lines (ARPE-19) can be cultured on ultrathin suspended electrospun nanofibre scaffolds (ENS) composed of hydrophobic polymer polyacrylonitrile (PAN) and a water-soluble aliphatic diamine, without (untreated) or with (treated) fluocinolone acetonide (FA).Cells survived and retained their characteristic morphology for up to 150 days with FA-treated ENS and manifested a morphological epithelial phenotype with expression of biomarkers critical for maintaining retinal physiological characteristics.This novel technique for producing culture substrates provides suitable hydrophilicity and a protective environment for prolonged RPE culture and has immense potential for subretinal transplantation.The findings indicate that FA-treated ENS is an excellent matrix Yellow Dock for retaining the differentiated and epithelial phenotype.